Question

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Anyone could help me please. Following questions are regarding Genetic Engineering.

1. Why in nature, high copy number result in underexpression, not overexpression?

2. How to make the dsDNA in gel become ss before transferring it onto nylon membrane?

3. How to estimate the weight of band in gel eletrophoresis?

4. What is the importance of plant catalase intron in the GUS gene of   pCAMBIA 2301?

Thanks!

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